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6 diamidino 2 phenylindole  (Beyotime)


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    Structured Review

    Beyotime 6 diamidino 2 phenylindole
    6 Diamidino 2 Phenylindole, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+with+dapi/Beyo3D+DAPI+Staining+Solution/pm42908353-49-46-47
    Average 99 stars, based on 1 article reviews
    6 diamidino 2 phenylindole - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: α -Ketoglutarate protects against cartilage damage via epigenetically driven metabolic reprogramming in osteoarthritis models
    Article Snippet: Immunofluorescence staining was carried out by incubation overnight at 4°C with antibodies against COL1A1 (Servicebio, GB11022-1; 1:200), Aspn (Affinity, DF13642; 1:100), Cilp (Huabio, ER1906-32; 1:100), Clu (Proteintech, 66109-1-Ig; 1:100), and Cnmd (Affinity, DF13715; 1:100). .. Alexa Fluor 594– or Cy3-conjugated secondary antibodies (Invitrogen) were added for 30 minutes, followed by staining with DAPI (Beyotime). .. Images were acquired with CaseViewer 2.3 scanning software (Servicebio), and analyzed with ImageJ software (NIH; version 1.8.0_112).

    Article Title: Exploring the role of RPS2 in muscle-invasive bladder cancer progression and resistance: A nanovesicle-based therapeutic approach
    Article Snippet: Newly synthesized RNA was labeled using the Cell-Light EU RNA Imaging Kit (C10316; RiboBio, Guangzhou, China). .. Cells were incubated with EU for 3h, followed by nuclear staining with DAPI (C1002; Beyotime Biotechnology) for 5 min. Fluorescence images were acquired with a fluorescence microscope (FV-1000/ES; Olympus), and 6–10 random fields were analyzed per sample. ..

    Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism
    Article Snippet: After blocking in 3 % BSA for half an hour, osteoblasts were incubated with a primary antibody anti -LC3 (1:500, 14600-1-AP, Proteintech, USA) at 4 °C overnight. .. Afterward, incubation with a secondary antibody Goat Anti-Rabbit Alexa Flour 488 (1:300, ab150077, Abcam, UK) was conducted at room temperature for 1 h. After staining with DAPI (C1006, Beyotime), the fluorescence was observed under a fluorescence microscope. ..

    Article Title: Acinetobacter baumannii inhibits the PLK2/FoxO axis to induce oxidative stress and cell barrier dysfunction
    Article Snippet: Cells were then permeabilized with 0.1% Triton X-100 (Solarbio, T8200) for 10 min and blocked with 5% BSA (Biofroxx, 4240GR100) for 1 h. The primary antibodies were added, and the cells were incubated overnight on a shaker at 4°C. .. The following day, the cells were incubated with fluorescent secondary antibodies, followed by staining with DAPI (Beyotime). .. Signals were visualized under an inverted fluorescence microscope and quantified using ImageJ.

    Article Title: The HKDC1-ASS1-ACSBG2 axis reprograms lipid metabolism to drive therapeutic resistance in hepatocellular carcinoma
    Article Snippet: .. After three washes with PBST, the cells were incubated with fluorescent secondary antibodies (Beyotime, Shanghai, China) for 1 h, followed by nuclear staining with DAPI (Beyotime, Shanghai, China). ..

    Article Title: ROS-responsive Prussian blue nanoplatform for synergistic myocardial ischemia/reperfusion injury therapy via EGCG delivery and DJ-1 pathway regulation.
    Article Snippet: Effective management of myocardial ischemia/reperfusion (I/R) injury remains a clinical challenge.. Epigallocatechin gallate (EGCG), a natural antioxidant, exhibits therapeutic potential in I/R models; however, it is limited by poor bioavailability and inadequate targeted delivery.. Therefore, using a synergistic therapeutic strategy, we designed a nanoplatform (PB@PEI@FPBA-EGCG NPs, referred to as PPFE NPs) to address these limitations.

    Article Title: After Traumatic Brain Injury, EPHA4 Enhances Endoplasmic Reticulum Stress to Promote M1 Microglial Polarization Through the MAPK Signaling Pathway
    Article Snippet: After the cell culture was complete, the medium was discarded, and the cells were fixed with 4% paraformaldehyde for 20 min. Then, the cells were incubated with primary antibodies against iNOS (1:200; 22226‐1‐AP; Proteintech, China) and Arg1 (1:200; 16001‐1‐AP; Proteintech, China) at 4°C overnight. .. Afterward, the corresponding fluorescent secondary antibody was incubated at room temperature for 2 h. After nuclear staining with DAPI (C1002; Beyotime, China) for 15 min, the cells were observed, and images were captured under a fluorescence microscope. .. The mean fluorescence intensity was quantified using ImageJ software.

    Article Title: Combined strategy of dual-module cerium nanosystem composite extracellular vesicles regulate ROS in the tissue microenvironment to promote periodontitis recovery
    Article Snippet: .. Cells were incubated with Vari Fluor 594-Phalloidin or Vari Fluor 488-Phalloidin (MCE) in the dark for 20 min, followed by nuclear staining with DAPI (P0131, Beyotime Biotechnology, China) for 10 min. Observations were performed using a confocal laser scanning microscope (CLSM; Olympus FV3000, Japan). .. The reactive oxygen species (ROS) detection kit (Beyotime Biotechnology, China) was used to evaluate the oxidative stress level in Raw264.7 macrophages.

    Incubation:

    Article Title: Exploring the role of RPS2 in muscle-invasive bladder cancer progression and resistance: A nanovesicle-based therapeutic approach
    Article Snippet: Newly synthesized RNA was labeled using the Cell-Light EU RNA Imaging Kit (C10316; RiboBio, Guangzhou, China). .. Cells were incubated with EU for 3h, followed by nuclear staining with DAPI (C1002; Beyotime Biotechnology) for 5 min. Fluorescence images were acquired with a fluorescence microscope (FV-1000/ES; Olympus), and 6–10 random fields were analyzed per sample. ..

    Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism
    Article Snippet: After blocking in 3 % BSA for half an hour, osteoblasts were incubated with a primary antibody anti -LC3 (1:500, 14600-1-AP, Proteintech, USA) at 4 °C overnight. .. Afterward, incubation with a secondary antibody Goat Anti-Rabbit Alexa Flour 488 (1:300, ab150077, Abcam, UK) was conducted at room temperature for 1 h. After staining with DAPI (C1006, Beyotime), the fluorescence was observed under a fluorescence microscope. ..

    Article Title: Acinetobacter baumannii inhibits the PLK2/FoxO axis to induce oxidative stress and cell barrier dysfunction
    Article Snippet: Cells were then permeabilized with 0.1% Triton X-100 (Solarbio, T8200) for 10 min and blocked with 5% BSA (Biofroxx, 4240GR100) for 1 h. The primary antibodies were added, and the cells were incubated overnight on a shaker at 4°C. .. The following day, the cells were incubated with fluorescent secondary antibodies, followed by staining with DAPI (Beyotime). .. Signals were visualized under an inverted fluorescence microscope and quantified using ImageJ.

    Article Title: The HKDC1-ASS1-ACSBG2 axis reprograms lipid metabolism to drive therapeutic resistance in hepatocellular carcinoma
    Article Snippet: .. After three washes with PBST, the cells were incubated with fluorescent secondary antibodies (Beyotime, Shanghai, China) for 1 h, followed by nuclear staining with DAPI (Beyotime, Shanghai, China). ..

    Article Title: ROS-responsive Prussian blue nanoplatform for synergistic myocardial ischemia/reperfusion injury therapy via EGCG delivery and DJ-1 pathway regulation.
    Article Snippet: Effective management of myocardial ischemia/reperfusion (I/R) injury remains a clinical challenge.. Epigallocatechin gallate (EGCG), a natural antioxidant, exhibits therapeutic potential in I/R models; however, it is limited by poor bioavailability and inadequate targeted delivery.. Therefore, using a synergistic therapeutic strategy, we designed a nanoplatform (PB@PEI@FPBA-EGCG NPs, referred to as PPFE NPs) to address these limitations.

    Article Title: After Traumatic Brain Injury, EPHA4 Enhances Endoplasmic Reticulum Stress to Promote M1 Microglial Polarization Through the MAPK Signaling Pathway
    Article Snippet: After the cell culture was complete, the medium was discarded, and the cells were fixed with 4% paraformaldehyde for 20 min. Then, the cells were incubated with primary antibodies against iNOS (1:200; 22226‐1‐AP; Proteintech, China) and Arg1 (1:200; 16001‐1‐AP; Proteintech, China) at 4°C overnight. .. Afterward, the corresponding fluorescent secondary antibody was incubated at room temperature for 2 h. After nuclear staining with DAPI (C1002; Beyotime, China) for 15 min, the cells were observed, and images were captured under a fluorescence microscope. .. The mean fluorescence intensity was quantified using ImageJ software.

    Article Title: Combined strategy of dual-module cerium nanosystem composite extracellular vesicles regulate ROS in the tissue microenvironment to promote periodontitis recovery
    Article Snippet: .. Cells were incubated with Vari Fluor 594-Phalloidin or Vari Fluor 488-Phalloidin (MCE) in the dark for 20 min, followed by nuclear staining with DAPI (P0131, Beyotime Biotechnology, China) for 10 min. Observations were performed using a confocal laser scanning microscope (CLSM; Olympus FV3000, Japan). .. The reactive oxygen species (ROS) detection kit (Beyotime Biotechnology, China) was used to evaluate the oxidative stress level in Raw264.7 macrophages.

    Fluorescence:

    Article Title: Exploring the role of RPS2 in muscle-invasive bladder cancer progression and resistance: A nanovesicle-based therapeutic approach
    Article Snippet: Newly synthesized RNA was labeled using the Cell-Light EU RNA Imaging Kit (C10316; RiboBio, Guangzhou, China). .. Cells were incubated with EU for 3h, followed by nuclear staining with DAPI (C1002; Beyotime Biotechnology) for 5 min. Fluorescence images were acquired with a fluorescence microscope (FV-1000/ES; Olympus), and 6–10 random fields were analyzed per sample. ..

    Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism
    Article Snippet: After blocking in 3 % BSA for half an hour, osteoblasts were incubated with a primary antibody anti -LC3 (1:500, 14600-1-AP, Proteintech, USA) at 4 °C overnight. .. Afterward, incubation with a secondary antibody Goat Anti-Rabbit Alexa Flour 488 (1:300, ab150077, Abcam, UK) was conducted at room temperature for 1 h. After staining with DAPI (C1006, Beyotime), the fluorescence was observed under a fluorescence microscope. ..

    Article Title: After Traumatic Brain Injury, EPHA4 Enhances Endoplasmic Reticulum Stress to Promote M1 Microglial Polarization Through the MAPK Signaling Pathway
    Article Snippet: After the cell culture was complete, the medium was discarded, and the cells were fixed with 4% paraformaldehyde for 20 min. Then, the cells were incubated with primary antibodies against iNOS (1:200; 22226‐1‐AP; Proteintech, China) and Arg1 (1:200; 16001‐1‐AP; Proteintech, China) at 4°C overnight. .. Afterward, the corresponding fluorescent secondary antibody was incubated at room temperature for 2 h. After nuclear staining with DAPI (C1002; Beyotime, China) for 15 min, the cells were observed, and images were captured under a fluorescence microscope. .. The mean fluorescence intensity was quantified using ImageJ software.

    Microscopy:

    Article Title: Exploring the role of RPS2 in muscle-invasive bladder cancer progression and resistance: A nanovesicle-based therapeutic approach
    Article Snippet: Newly synthesized RNA was labeled using the Cell-Light EU RNA Imaging Kit (C10316; RiboBio, Guangzhou, China). .. Cells were incubated with EU for 3h, followed by nuclear staining with DAPI (C1002; Beyotime Biotechnology) for 5 min. Fluorescence images were acquired with a fluorescence microscope (FV-1000/ES; Olympus), and 6–10 random fields were analyzed per sample. ..

    Article Title: Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism
    Article Snippet: After blocking in 3 % BSA for half an hour, osteoblasts were incubated with a primary antibody anti -LC3 (1:500, 14600-1-AP, Proteintech, USA) at 4 °C overnight. .. Afterward, incubation with a secondary antibody Goat Anti-Rabbit Alexa Flour 488 (1:300, ab150077, Abcam, UK) was conducted at room temperature for 1 h. After staining with DAPI (C1006, Beyotime), the fluorescence was observed under a fluorescence microscope. ..

    Article Title: After Traumatic Brain Injury, EPHA4 Enhances Endoplasmic Reticulum Stress to Promote M1 Microglial Polarization Through the MAPK Signaling Pathway
    Article Snippet: After the cell culture was complete, the medium was discarded, and the cells were fixed with 4% paraformaldehyde for 20 min. Then, the cells were incubated with primary antibodies against iNOS (1:200; 22226‐1‐AP; Proteintech, China) and Arg1 (1:200; 16001‐1‐AP; Proteintech, China) at 4°C overnight. .. Afterward, the corresponding fluorescent secondary antibody was incubated at room temperature for 2 h. After nuclear staining with DAPI (C1002; Beyotime, China) for 15 min, the cells were observed, and images were captured under a fluorescence microscope. .. The mean fluorescence intensity was quantified using ImageJ software.

    Confocal:

    Article Title: Combined strategy of dual-module cerium nanosystem composite extracellular vesicles regulate ROS in the tissue microenvironment to promote periodontitis recovery
    Article Snippet: .. Cells were incubated with Vari Fluor 594-Phalloidin or Vari Fluor 488-Phalloidin (MCE) in the dark for 20 min, followed by nuclear staining with DAPI (P0131, Beyotime Biotechnology, China) for 10 min. Observations were performed using a confocal laser scanning microscope (CLSM; Olympus FV3000, Japan). .. The reactive oxygen species (ROS) detection kit (Beyotime Biotechnology, China) was used to evaluate the oxidative stress level in Raw264.7 macrophages.

    Laser-Scanning Microscopy:

    Article Title: Combined strategy of dual-module cerium nanosystem composite extracellular vesicles regulate ROS in the tissue microenvironment to promote periodontitis recovery
    Article Snippet: .. Cells were incubated with Vari Fluor 594-Phalloidin or Vari Fluor 488-Phalloidin (MCE) in the dark for 20 min, followed by nuclear staining with DAPI (P0131, Beyotime Biotechnology, China) for 10 min. Observations were performed using a confocal laser scanning microscope (CLSM; Olympus FV3000, Japan). .. The reactive oxygen species (ROS) detection kit (Beyotime Biotechnology, China) was used to evaluate the oxidative stress level in Raw264.7 macrophages.

    Confocal Laser Scanning Microscopy:

    Article Title: Combined strategy of dual-module cerium nanosystem composite extracellular vesicles regulate ROS in the tissue microenvironment to promote periodontitis recovery
    Article Snippet: .. Cells were incubated with Vari Fluor 594-Phalloidin or Vari Fluor 488-Phalloidin (MCE) in the dark for 20 min, followed by nuclear staining with DAPI (P0131, Beyotime Biotechnology, China) for 10 min. Observations were performed using a confocal laser scanning microscope (CLSM; Olympus FV3000, Japan). .. The reactive oxygen species (ROS) detection kit (Beyotime Biotechnology, China) was used to evaluate the oxidative stress level in Raw264.7 macrophages.



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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing